1. 圣淘沙名胜世界(新加坡)

    using a microwave instead of autoclave to sterilize bacterial culture media

    Joseph Shenekji

    Published: 2024-05-30 DOI: 10.17504/protocols.io.eq2lywwzmvx9/v1
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    Disclaimer

    this method should only used for routine culture, it is not advised for novel bacteria or any other important first culture.

    Abstract

    this protocol uses a microwave to sterilize and polymerize culture media for bacterial propagation in 3 minutes, the whole protocol can be done in 15-20 minutes without the need for an autoclave and waiting an hour for it to heat to 121°Cwhich can consume a lot of electricity and time, this method is straight forward and clean and gave good results of growth without pollution of non desired bacteria, it can be used for liquid cultures and solid cultures as well.

    Before start

    prepare your media and bottle and plates, and make sure to turn on the laminar flow hood 15 minutes ahead of culture.

    Steps

    adding the media powder

    1.

    measure the desired quantity of media powder you want with a sensitive scale, ex: 4g of Sampleinside a heat resistant glass bottle with a lid with proper volume (size 250ml).

    2.

    add the water you need, preferably distilled but tap water works fine for me, ex:100mL.

    3.

    close the lid tightly to create pressure, and mix by shaking in your hand for 0h 0m 30s until the powder is dissolved.

    4.

    put the media in the microwave n the highest degree for 0h 3m 0s, stop the microwave if you see bubbles appearing, let the bottle rest for few seconds then continue the process.

    5.

    you may need an extra minute to make sure everything is correct, the media will look yellow and transparent.

    6.

    take out the bottle with the media and let it cool under the flow of tap water, be sure to rotate the bottle under water until it becomes easy to touch with your hand, that means it reached 60-65°C.

    7.

    sterilize the bottle by rubbing alcohol on it, and take it inside the laminar flow hood.

    8.

    add your desired antibiotic like ampicillin ampicillin  ( 10µL ) to the media and shake inside the hood gently to spread it.

    9.

    pour the media inside petri dishes, about 10-15mL is enough, then wait for about 0h 15m 0s until it cools off and solidify.

    10.

    culture on your plates containing the sterilized media with the microwave method, then close the plates and place them in an incubator 37°C to grow your culture for 16-24 hours.

    results are here shown after 24 hours of growth

    11.

    you can visually see the solid LB agar plates with grown cultures and no pollution of other bacteria.

    LB agar plate sterilized and prepare in the microwave with bacterial growth PLYSs
    LB agar plate sterilized and prepare in the microwave with bacterial growth PLYSs
    12.

    i also tried a liquid LB broth and it gave good results with no pollution with very good growth.

    microwaved LB broth with PlySs culture it gave a very good density of turbidity from grwoth.
    microwaved LB broth with PlySs culture it gave a very good density of turbidity from grwoth.

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