Tutorial: using nanoneedles for intracellular delivery

Ciro Chiappini, Yaping Chen, Stella Aslanoglou, Anna Mariano, Valentina Mollo, Huanwen Mu, Enrica De Rosa, Gen He, Ennio Tasciotti, Xi Xie, Francesca Santoro, Wenting Zhao, Nicolas H. Voelcker, Roey Elnathan

Published: 2021-08-22 DOI: 10.1038/s41596-021-00600-7

nanoneedles

intracellular delivery

therapeutic nucleic acids

nanoinjection workflow

bioassays

AI 解读

Efficient and safe single-cell cloning of human pluripotent stem cells using the CEPT cocktail

Profiling native pulmonary basement membrane stiffness using atomic force microscopy

Genetic circuit design automation with Cello 2.0

Synthesis of siRNA nanoparticles to silence plaque-destabilizing gene in atherosclerotic lesional macrophages

Scalable dual-omics profiling with single-nucleus chromatin accessibility and mRNA expression sequencing 2 (SNARE-seq2)

A practical guide to scanning light-field microscopy with digital adaptive optics

Effective high-throughput isolation of fully human antibodies targeting infectious pathogens

Improved sampling and DNA extraction procedures for microbiome analysis in food-processing environments

MOF-derived nanoporous carbons with diverse tunable nanoarchitectures

Tutorial: a statistical genetics guide to identifying HLA alleles driving complex disease

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Intracellular delivery of advanced therapeutics, including biologicals and supramolecular agents, is complex because of the natural biological barriers that have evolved to protect the cell. Efficient delivery of therapeutic nucleic acids, proteins, pepti
Main
Nanoneedle devices
Cargoes
Biological systems
Interfacing
Evaluation
Concluding remarks
- References
References
ACKNOWLEDGMENTS

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Tutorial: using nanoneedles for intracellular delivery

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