Stress-free single-cell transcriptomic profiling and functional genomics of murine eosinophils

Costanza Borrelli, Alessandra Gurtner, Isabelle C. Arnold, Andreas E. Moor

Published: 2024-03-19 DOI: 10.1038/s41596-024-00967-3

CRISPR screening

single-cell RNA sequencing

eosinophils

granulocytes

intestinal subsets

AI 解读

Generation of mouse–human chimeric embryos

Genome-wide quantification of transcription factor binding at single-DNA-molecule resolution using methyl-transferase footprinting

Generation of CRISPR–Cas9-mediated genetic knockout human intestinal tissue–derived enteroid lines by lentivirus transduction and single-cell cloning

A puromycin-dependent activity-based sensing probe for histochemical staining of hydrogen peroxide in cells and animal tissues

Decoding personal biotic and abiotic airborne exposome

A clinically relevant model of focal embolic cerebral ischemia by thrombus and thrombolysis in rhesus monkeys

Bioengineering human intestinal mucosal grafts using patient-derived organoids, fibroblasts and scaffolds

Biomimetic tail-to-head terpene cyclizations using the resorcin[4]arene capsule catalyst

Neuroscience robotics for controlled induction and real-time assessment of hallucinations

Scanorama: integrating large and diverse single-cell transcriptomic datasets

Single-cell RNA sequencing (scRNA-seq)（单细胞RNA测序）

Data integration（数据集成）

Heterogeneity（异质性）

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Sections

Figures

References

Introduction
Materials
- Stress-free scRNASeq of tissue resident eosinophils
- Pooled CRISPRi screen in bone-marrow derived eosinophils
Procedure 1: single-cell profiling of tissue resident eosinophils
Procedure 2: pooled CRISPRi screen in bone-marrow derived eosinophils
Troubleshooting
Timing
- Single-cell profiling of tissue resident eosinophils
- Pooled CRISPRi screen in bone marrow-derived eosinophils
Anticipated results
- Reporting summary
Acknowledgements

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Stress-free single-cell transcriptomic profiling and functional genomics of murine eosinophils

Supplementary information

Supplementary Information

Reporting Summary

Supplementary Table 1

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