Principles of reproducible metabolite profiling of enriched lymphocytes in tumors and ascites from human ovarian cancer

Ryan D. Sheldon, Russell G. Jones, Ralph J. DeBerardinis, Marisa K. Kilgour, Sarah MacPherson, Lauren G. Zacharias, Jodi LeBlanc, Sindy Babinszky, Gabrielle Kowalchuk, Scott Parks, Phineas T. Hamilton, Peter H. Watson, Julian J. Lum

Published: 2022-08-18 DOI: 10.1038/s41596-022-00729-z

mass spectrometry

ovarian cancer

metabolite profiling

immune cells

cellular enrichment

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Identifying metabolites and delineating their immune-regulatory contribution in the tumor microenvironment is an area of intense study. Interrogating metabolites and metabolic networks among immune cell subsets and host cells from resected tissues and flu
Introduction
Materials for Procedure 1
Materials for Procedure 2
Materials for Procedure 3
Procedure 1: establishing a team and workflow for patient enrollment, biospecimen/data collection and storage of biospecimens
Procedure 2: cellular enrichment and metabolite profiling by MS
Procedure 3: high-dimensional flow cytometry for metabolic profiling
Timing
Anticipated results
References
ACKNOWLEDGMENTS

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Principles of reproducible metabolite profiling of enriched lymphocytes in tumors and ascites from human ovarian cancer

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