Monitoring genome-wide replication fork directionality by Okazaki fragment sequencing in mammalian cells

Sarah Kit Leng Lui, Sarah Keegan, Peter Tonzi, Malik Kahli, Yu-Hung Chen, Noor Chalhoub, Kate E. Coleman, David Fenyo, Duncan J. Smith, Tony T. Huang

Published: 2021-01-12 DOI: 10.1038/s41596-020-00454-5

Okazaki fragment sequencing

genome-wide replication

DNA replication dynamics

mammalian cells

replication fork directionality

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The ability to monitor DNA replication fork directionality at the genome-wide scale is paramount for a greater understanding of how genetic and environmental perturbations can impact replication dynamics in human cells. Here we describe a detailed protoco
Introduction
Materials
Procedure
Troubleshooting
Timing
Anticipated results
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References
ACKNOWLEDGMENTS
ACKNOWLEDGMENTS

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Monitoring genome-wide replication fork directionality by Okazaki fragment sequencing in mammalian cells

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