DNA yield estimation and custom DNA quality evaluation before shotgun metagenomic sequencing
Benoit Quinquis , Alexandre Famechon , nathalie.galleron, Victoria Meslier, Mathieu Almeida
Published: 2024-02-13 DOI: 10.17504/protocols.io.5qpvo3zzzv4o/v1
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Abstract
This protocol describes the procedures used for DNA quantification and a custom DNA quality evaluation before proceeding to shotgun metagenomic seqeuencing.
Before start
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Attachments
Quant-iT-DNA-BR-protocol.pdf DNA_yield_&_custom_quality_evaluation_for_shotgun_metagenomic_sequencing_MetaGenoPolis.pdf
Steps
DNA quantification
1.
using the DNA-binding fluorescent Quant-it dsDNA
BR assay
Custom DNA quality estimation
2.
Genomic 50 kb kit by capillary electrophoresis using the Fragment Analyzer, followed by a custom DNA quality analysis
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