Composable microfluidic spinning platforms for facile production of biomimetic perfusable hydrogel microtubes

Ruoxiao Xie, Zhe Liang, Yongjian Ai, Wenchen Zheng, Jialiang Xiong, Peidi Xu, Yupeng Liu, Mingyu Ding, Jianyi Gao, Jiaping Wang, Qionglin Liang

Published: 2020-12-13 DOI: 10.1038/s41596-020-00442-9

microfluidic devices

hydrogel microtubes

biomimetic

perfusion

tissue engineering

AI 解读

Efficient and safe single-cell cloning of human pluripotent stem cells using the CEPT cocktail

Profiling native pulmonary basement membrane stiffness using atomic force microscopy

Genetic circuit design automation with Cello 2.0

Synthesis of siRNA nanoparticles to silence plaque-destabilizing gene in atherosclerotic lesional macrophages

Scalable dual-omics profiling with single-nucleus chromatin accessibility and mRNA expression sequencing 2 (SNARE-seq2)

A practical guide to scanning light-field microscopy with digital adaptive optics

Effective high-throughput isolation of fully human antibodies targeting infectious pathogens

Improved sampling and DNA extraction procedures for microbiome analysis in food-processing environments

MOF-derived nanoporous carbons with diverse tunable nanoarchitectures

Tutorial: a statistical genetics guide to identifying HLA alleles driving complex disease

查看全部

Sections

Figures

References

Microtissues with specific structures and integrated vessels play a key role in maintaining organ functions. To recapitulate the invivo environment for tissue engineering and organ-on-a-chip purposes, it is essential to develop perfusable biomimetic micr
Introduction
Materials
Procedure
Troubleshooting
Timing
Anticipated results
- Reporting Summary
- References
References
ACKNOWLEDGMENTS

Liu, C. et al. Microfluidic fabrication of water-in-water droplets encapsulated in hydrogel microfibers. Chin. Chem. Lett. 30, 457–460 (2019).
Van Vlierberghe, S., Dubruel, P. & Schacht, E. Biopolymer-based hydrogels as scaffolds for tissue engineering applications: a review. Biomacromolecules 12, 1387–1408 (2011).
Zhang, Y. S. & Khademhosseini, A. Advances in engineering hydrogels. Science 356, eaaf3627 (2017).
Jacob, M., Chappell, D. & Becker, B. F. Regulation of blood flow and volume exchange across the microcirculation. Crit. Care 20, 319 (2016).
Pellegata, A. F., Tedeschi, A. M. & De Coppi, P. Whole organ tissue vascularization: engineering the tree to develop the fruits. Front. Bioeng. Biotechnol. 6, 56 (2018).
de Bono, B., Grenon, P., Baldock, R. & Hunter, P. Functional tissue units and their primary tissue motifs in multi-scale physiology. J. Biomed. Semant. 4, 22 (2013).
Pollak, M. R., Quaggin, S. E., Hoenig, M. P. & Dworkin, L. D. The glomerulus: the sphere of influence. Clin. J. Am. Soc. Nephrol. 9, 1461–1469 (2014).
Weibel, E. R. Morphological basis of alveolar-capillary gas exchange. Physiol. Rev. 53, 419–495 (1973).
Rappaport, A. M. & Wilson, W. D. The stuctural and functional unit in the human liver (liver acinus). Anat. Rec. 130, 673–689 (1958).
Weber, G. M., Ju, Y. & Börner, K. Considerations for using the vasculature as a coordinate system to map all the cells in the human body. Front. Cardiovasc. Med. 7, 29 (2020).
Yang, J., Yu, L. X., Rennie, M. Y., Sled, J. G. & Henkelman, R. M. Comparative structural and hemodynamic analysis of vascular trees. Am. J. Physiol. Heart Circ. Physiol. 298, H1249–H1259 (2010).
Niu, L. et al. Influence of vascular geometry on local hemodynamic parameters: phantom and small rodent study. Biomed. Eng. Online 17, 30 (2018).
Kim, B. J. et al. Basilar artery plaque and pontine infarction and vascular geometry. J. Stroke 20, 92 (2018).
Pijnenborg, R., Vercruysse, L. & Hanssens, M. The uterine spiral arteries in human pregnancy: facts and controversies. Placenta 27, 939–958 (2006).
Tan, J., Shah, S., Thomas, A., Ou-Yang, H. D. & Liu, Y. The influence of size, shape and vessel geometry on nanoparticle distribution. Microfluid. Nanofluidics 14, 77–87 (2013).
Rouwkema, J. & Khademhosseini, A. Vascularization and angiogenesis in tissue engineering: beyond creating static networks. Trends Biotechnol. 34, 733–745 (2016).
Ai, Y., Zhang, F., Wang, C., Xie, R. & Liang, Q. Recent progress in lab-on-a-chip for pharmaceutical analysis and pharmacological/toxicological test. TrAC Trends Anal. Chem. 117, 215–230 (2019).
Xie, R., Zheng, W., Guan, L., Ai, Y. & Liang, Q. Engineering of hydrogel materials with perfusable microchannels for building vascularized tissues. Small 15, 1902838 (2019).
Zheng, F. et al. Organ-on-a-chip systems: microengineering to biomimic living systems. Small 12, 2253–2282 (2016).
Khademhosseini, A. & Langer, R. Microengineered hydrogels for tissue engineering. Biomaterials 28, 5087–5092 (2007).
Li, L. et al. Simultaneous assay of oxygen-dependent cytotoxicity and genotoxicity of anticancer drugs on an integrated microchip. Anal. Chem. 90, 11899–11907 (2018).
Wang, W., Li, L., Ding, M., Luo, G. & Liang, Q. A microfluidic hydrogel chip with orthogonal dual gradients of matrix stiffness and oxygen for cytotoxicity test. BioChip J. 12, 93–101 (2018).
Liang, Z. et al. Double-network hydrogel with tunable mechanical performance and biocompatibility for the fabrication of stem cells-encapsulated fibers and 3D assemble. Sci. Rep. 6, 33462 (2016).
Shin, Y. et al. Microfluidic assay for simultaneous culture of multiple cell types on surfaces or within hydrogels. Nat. Protoc. 7, 1247–1259 (2012).
Liang, Z., Liu, Y., Zhang, F., Ai, Y. & Liang, Q. Dehydration-triggered shape morphing based on asymmetric bubble hydrogel microfibers. Soft Matter 14, 6623–6626 (2018).
Liu, Y. et al. Hydrogel microfibers with perfusable folded channels for tissue constructs with folded morphology. RSC Adv. 8, 23475–23480 (2018).
Xie, R. et al. h-FIBER: microfluidic topographical hollow fiber for studies of glomerular filtration barrier. ACS Cent. Sci. 6, 903–912 (2020).
Xie, R. et al. Necklace-like microfibers with variable knots and perfusable channels fabricated by an oil-free microfluidic spinning process. Adv. Mater. 30, 1705082 (2018).
Xu, P. et al. Bioinspired microfibers with embedded perfusable helical channels. Adv. Mater. 29, 1701664 (2017).
Luo, X., Su, P., Zhang, W. & Raston, C. L. Microfluidic devices in fabricating nano or micromaterials for biomedical applications. Adv. Mater. Technol. 4, 1900488 (2019).
Jun, Y., Kang, E., Chae, S. & Lee, S. Microfluidic spinning of micro-and nano-scale fibers for tissue engineering. Lab Chip 14, 2145–2160 (2014).
Zhu, A. & Guo, M. Microfluidic controlled mass-transfer and buckling for easy fabrication of polymeric helical fibers. Macromol. Rapid Commun. 37, 426–432 (2016).
Hwang, C. M. et al. Controlled cellular orientation on PLGA microfibers with defined diameters. Biomed. Microdevices 11, 739–746 (2009).
Bai, Z., Mendoza Reyes, J. M., Montazami, R. & Hashemi, N. On-chip development of hydrogel microfibers from round to square/ribbon shape. J. Mater. Chem. A 2, 4878 (2014).
Cho, S., Shim, T. S. & Yang, S. High-throughput optofluidic platforms for mosaicked microfibers toward multiplex analysis of biomolecules. Lab Chip 12, 3676 (2012).
Lee, K. H. et al. Microfluidic synthesis of pure chitosan microfibers for bio-artificial liver chip. Lab Chip 10, 1328 (2010).
Onoe, H. et al. Metre-long cell-laden microfibres exhibit tissue morphologies and functions. Nat. Mater. 12, 584–590 (2013).
Hu, M. et al. Hydrodynamic spinning of hydrogel fibers. Biomaterials 31, 863–869 (2010).
Wang, Y. et al. A 3D construct of the intestinal canal with wrinkle morphology on a centrifugation configuring microfluidic chip. Biofabrication 11, 045001 (2019).
Ma, L. et al. Design and fabrication of a liver-on-a-chip platform for convenient, highly efficient, and safe in situ perfusion culture of 3D hepatic spheroids. Lab Chip 18, 2547–2562 (2018).
Kang, E. et al. Digitally tunable physicochemical coding of material composition and topography in continuous microfibres. Nat. Mater. 10, 877–883 (2011).
Yu, Y. et al. Simple spinning of heterogeneous hollow microfibers on chip. Adv. Mater. 28, 6649–6655 (2016).
Lee, K. H., Shin, S. J., Park, Y. & Lee, S. Synthesis of cell-laden alginate hollow fibers using microfluidic chips and microvascularized tissue-engineering applications. Small 5, 1264–1268 (2009).
Jeong, W. et al. Hydrodynamic microfabrication via “on the fly” photopolymerization of microscale fibers and tubes. Lab Chip 4, 576–580 (2004).
Yu, Y., Shang, L., Guo, J., Wang, J. & Zhao, Y. Design of capillary microfluidics for spinning cell-laden microfibers. Nat. Protoc. 13, 2557–2579 (2018).
Meng, Z. et al. Microfluidic generation of hollow Ca-alginate microfibers. Lab Chip 16, 2673–2681 (2016).
Gao, C., Wang, X., Du, Q., Tang, J. & Jiang, J. Generation of perfusable hollow calcium alginate microfibers with a double co-axial flow capillary microfluidic device. Biomicrofluidics 13, 064108 (2019).
Whitesides, G. M., Ostuni, E., Takayama, S., Jiang, X. & Ingber, D. E. Soft lithography in biology and biochemistry. Annu. Rev. Biomed. Eng. 3, 335–373 (2001).
Liau, B., Bursac, N., Bian, W. & Badie, N. Mesoscopic hydrogel molding to control the 3D geometry of bioartificial muscle tissues. Nat. Protoc. 4, 1522–1534 (2009).
Qiu, Y. et al. Microvasculature-on-a-chip for the long-term study of endothelial barrier dysfunction and microvascular obstruction in disease. Nat. Biomed. Eng. 2, 453–463 (2018).
Choi, N. W. et al. Microfluidic scaffolds for tissue engineering. Nat. Mater. 6, 908 (2007).
Chrobak, K. M., Potter, D. R. & Tien, J. Formation of perfused, functional microvascular tubes in vitro. Microvasc. Res. 71, 185–196 (2006).
Li, X. et al. Engineering of microscale vascularized fat that responds to perfusion with lipoactive hormones. Biofabrication 11, 014101 (2018).
Polacheck, W. J., Kutys, M. L., Tefft, J. B. & Chen, C. S. Microfabricated blood vessels for modeling the vascular transport barrier. Nat. Protoc. 14, 1425–1454 (2019).
Mori, N., Morimoto, Y. & Takeuchi, S. Skin integrated with perfusable vascular channels on a chip. Biomaterials 116, 48–56 (2017).
Mannino, R. G. et al. 3D microvascular model recapitulates the diffuse large B-cell lymphoma tumor microenvironment in vitro. Lab Chip 17, 407–414 (2017).
Golden, A. P. & Tien, J. Fabrication of microfluidic hydrogels using molded gelatin as a sacrificial element. Lab Chip 7, 720–725 (2007).
Tocchio, A. et al. Versatile fabrication of vascularizable scaffolds for large tissue engineering in bioreactor. Biomaterials 45, 124–131 (2015).
Wang, X. et al. Engineering interconnected 3D vascular networks in hydrogels using molded sodium alginate lattice as the sacrificial template. Lab Chip 14, 2709–2716 (2014).
Hu, M. et al. Facile engineering of long-term culturable ex vivo vascularized tissues using biologically derived matrices. Adv. Healthcare Mater. 7, 1800845 (2018).
Miller, J. S. et al. Rapid casting of patterned vascular networks for perfusable engineered three-dimensional tissues. Nat. Mater. 11, 768 (2012).
Kolesky, D. B., Homan, K. A., Skylar-Scott, M. A. & Lewis, J. A. Three-dimensional bioprinting of thick vascularized tissues. Proc. Natl Acad. Sci. USA 113, 3179–3184 (2016).
Kang, H. et al. A 3D bioprinting system to produce human-scale tissue constructs with structural integrity. Nat. Biotechnol. 34, 312 (2016).
Kolesky, D. B. et al. 3D bioprinting of vascularized, heterogeneous cell-laden tissue constructs. Adv. Mater. 26, 3124–3130 (2014).
Hinton, T. J. et al. Three-dimensional printing of complex biological structures by freeform reversible embedding of suspended hydrogels. Sci. Adv. 1, 1500758 (2015).
Song, K. H., Highley, C. B., Rouff, A. & Burdick, J. A. Complex 3D-printed microchannels within cell‐degradable hydrogels. Adv. Funct. Mater. 28, 1801331 (2018).
Wu, W., DeConinck, A. & Lewis, J. A. Omnidirectional printing of 3D microvascular networks. Adv. Mater. 23, 178–183 (2011).
Highley, C. B., Rodell, C. B. & Burdick, J. A. Direct 3D printing of shear-thinning hydrogels into self‐healing hydrogels. Adv. Mater. 27, 5075–5079 (2015).
Lee, A. et al. 3D bioprinting of collagen to rebuild components of the human heart. Science 365, 482–487 (2019).
Bhattacharjee, T. et al. Writing in the granular gel medium. Sci. Adv. 1, 1500655 (2015).
Skylar-Scott, M. A. et al. Biomanufacturing of organ-specific tissues with high cellular density and embedded vascular channels. Sci. Adv. 5, 2459 (2019).
Grigoryan, B. et al. Multivascular networks and functional intravascular topologies within biocompatible hydrogels. Science 364, 458–464 (2019).
Xue, D. et al. Projection-based 3D printing of cell patterning scaffolds with multiscale channels. ACS Appl. Mater. Interfaces 10, 19428–19435 (2018).
Zhang, R. & Larsen, N. B. Stereolithographic hydrogel printing of 3D culture chips with biofunctionalized complex 3D perfusion networks. Lab Chip 17, 4273–4282 (2017).
Zhang, A. P. et al. Rapid fabrication of complex 3D extracellular microenvironments by dynamic optical projection stereolithography. Adv. Mater. 24, 4266–4270 (2012).
Sun, W. et al. The bioprinting roadmap. Biofabrication 12, 022002 (2020).
Tian, Y. et al. Large-scale water collection of bioinspired cavity-microfibers. Nat. Commun. 8, 1–9 (2017).
Ji, X., Guo, S., Zeng, C., Wang, C. & Zhang, L. Continuous generation of alginate microfibers with spindle-knots by using a simple microfluidic device. RSC Adv. 5, 2517–2522 (2015).
Cheng, Y. et al. Bioinspired multicompartmental microfibers from microfluidics. Adv. Mater. 26, 5184–5190 (2014).
Kang, E. et al. Microfluidic spinning of flat alginate fibers with grooves for cell-aligning scaffolds. Adv. Mater. 24, 4271–4277 (2012).
Mulivor, A.W., Shevkoplyas, S., Sun, C., Bitensky, M.W. & Munn, L.L. Blood dynamics in microfabricated vessel networks. in Proceedings of the 2006 NSTI Nanotechnology Conference and Trade Show Vol. 2 (NSTI Nanotechnology, 2006).
Han, H. Twisted blood vessels: symptoms, etiology and biomechanical mechanisms. J. Vasc. Res. 49, 185–197 (2012).
Pedersen-Bjergaard, S. & Rasmussen, K. E. Liquid-phase microextraction with porous hollow fibers, a miniaturized and highly flexible format for liquid–liquid extraction. J. Chromatogr. A 1184, 132–142 (2008).
Wang, X., Feng, J. I., Bai, Y., Zhang, Q. & Yin, Y. Synthesis, properties, and applications of hollow micro-/nanostructures. Chem. Rev. 116, 10983–11060 (2016).
Ai, Y., Xie, R., Xiong, J. & Liang, Q. Microfluidics for bio-synthesizing: from droplets and vesicles to artificial cells. Small 15, 1903940 (2019).
Dong, R., Liu, Y., Mou, L., Deng, J. & Jiang, X. Microfluidics-based biomaterials and biodevices. Adv. Mater. 31, 1805033 (2019).

Composable microfluidic spinning platforms for facile production of biomimetic perfusable hydrogel microtubes

Supplementary information

Supplementary Information

Reporting Summary

Supplementary Data 1

推荐阅读