Comparative analysis of assays to measure CAR T-cell-mediated cytotoxicity

Stefan Kiesgen, John C. Messinger, Navin K. Chintala, Zachary Tano, Prasad S. Adusumilli

Published: 2021-02-14 DOI: 10.1038/s41596-020-00467-0

CAR T-cell

cytotoxicity assay

immune effector cells

antitumor efficacy

FDA directives

AI 解读

Generation of mouse–human chimeric embryos

Genome-wide quantification of transcription factor binding at single-DNA-molecule resolution using methyl-transferase footprinting

Generation of CRISPR–Cas9-mediated genetic knockout human intestinal tissue–derived enteroid lines by lentivirus transduction and single-cell cloning

A puromycin-dependent activity-based sensing probe for histochemical staining of hydrogen peroxide in cells and animal tissues

Decoding personal biotic and abiotic airborne exposome

A clinically relevant model of focal embolic cerebral ischemia by thrombus and thrombolysis in rhesus monkeys

Bioengineering human intestinal mucosal grafts using patient-derived organoids, fibroblasts and scaffolds

Biomimetic tail-to-head terpene cyclizations using the resorcin[4]arene capsule catalyst

Neuroscience robotics for controlled induction and real-time assessment of hallucinations

Scanorama: integrating large and diverse single-cell transcriptomic datasets

Single-cell RNA sequencing (scRNA-seq)（单细胞RNA测序）

Data integration（数据集成）

Heterogeneity（异质性）

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Sections

Figures

References

The antitumor efficacy of genetically engineered ‘living drugs’, including chimeric antigen receptor and T-cell receptor Tcells, is influenced by their activation, proliferation, inhibition, and exhaustion. A sensitive and reproducible cy
Main
51Cr assay
BLI luciferase assay
Impedance-based assay
Flow assay
Alternative assays
Advanced invitro antitumor efficacy assays
Potency assays for clinical cell therapy products
Assessment of cell therapies beyond cytotoxicity
Discussion
References
ACKNOWLEDGMENTS

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Comparative analysis of assays to measure CAR T-cell-mediated cytotoxicity

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