This protocol aims to assist those new to qPCR data analysis and those who seek to escape the closed-source, proprietary software tied to their qPCR instrumentation.

Here we describe how to assess the quality of qPCR data and qPCR assays using:

• CFX Maestro 2.3, the software native to Bio-Rad qPCR instruments,

• Microsoft Excel version 2404,

• a Bayesian inference Using Gibbs Sampling (BUGS) script presented by PJ Schmidt et al.,

• the 'LOD calculator' R-script, and

• the ‘qpcR’ R software package

03_computers_20240721.pdf